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Figure 4 | Virology Journal

Figure 4

From: A Leu to Ile but not Leu to Val change at HIV-1 reverse transcriptase codon 74 in the background of K65R mutation leads to an increased processivity of K65R+L74I enzyme and a replication competent virus

Figure 4

Comparison of R→K reversion dynamics at RT codon 65 for K65R+L74V and K65R+L74I viruses by direct and population sequencing. PHA-stimulated PBM cells were infected with equivalent amount (0.01 IU) of the 293 cells-derived doubly mutant viruses. Infectious viruses were sequenced at each time point shown and % R→K reversion was calculated. Population cloning of RT PCR product was performed and 20 independent clones for days 19, 24 and 28 were sequenced. A significant decrease in the reversion was observed with K65R+L74I viruses in comparison to K65R+L74V viruses. No reversion was observed at codon 74 in both double mutant viruses. Reversion data shows that the RT containing isoleucine change at RT codon 74 is much more stable than that with valine change in the background of K65R mutation.

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Virology Journal

ISSN: 1743-422X