Table 1 Identification of the 3 biallelic IL-10 promoter polymorphisms by PCR amplification and restriction enzyme digest
IL-10 promoter polymorphism (from transcription initiation site) | -592 (A/C) | -819 (T/C) | -1082 (G/A) |
|---|---|---|---|
PCR primers | 5' cct agg tca cag tga cgt gg 3' 5' ggt gag cac tac ctg act agc 3' | 5' tca ttc tat gtg ctg gag atg g 3' 5' tgg ggg aag tgg gta aga gt 3' | 5' ctc gct gca acc caa ctg gc 3' 5' ctc gct gca acc caa ctg gc 3' |
PCR product size (bp) | 419 | 419 | 139 |
Restriction enzyme | Rsa 1 | Mae III | Mnl |
Digest interpretation | Cuts the rarer A allele to generate 176- and 236-bp fragments | Cuts the more common C allele to generate242- and 141-bp fragments | direct sequencing |