Figure 3
From: Monoclonal antibody-based serological methods for detection of Cucumber green mottle mosaic virus
Sensitivity analyses of ACP-ELISA (A) and TAS-ELISA (B). A: The sensitivities of ACP-ELISA and TAS-ELISA in the detection of purified CGMMV. The purified virus preparation was two-fold diluted in PBS buffer. CK: healthy tissues preparation was two-fold diluted in PBS buffer. The dilution endpoint of ACP-ELISA and TAS-ELISA were 1:1024000 and 4096000, corresponding to an equivalent of 0.16 ng and 0.04 ng of purified viruses respectively. B: The sensitivities of ACP-ELISA and TAS-ELISA in the detection of CGMMV in infected leaf extracts. CGMMV-infected leaf extracts were two-fold diluted in PBS buffer. CK: healthy leaf extracts were two-fold diluted in PBS buffer. The dilution endpoint of ACP-ELISA and TAS-ELISA were 1:5120 and 1:20480 (w/v, g mL-1), respectively.