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Table 1 Summary of HIV/HA-mediated relative infectivity and HA and NA activity

From: Identification of critical residues of influenza neuraminidase in viral particle release

NA construct

Luciferase activityª

HA U/ml

4-MUNANA Release (a.u.)

 

293T cells

A549 cells

  

NAH

1.7 (±0.03) × 107

6.9 (±0.5) × 106

64

9.3 (±0.41) × 104

   E119V

8.1 (±2.10) × 105

1.2 (±0.1) × 103

0

8.3 (±0.51) × 103

   N234Y

1.7 (±0.02) × 106

4.3 (±1.00) × 105

16

2.5 (±0.04) × 104

   M241R

2.7 (±0.10) × 105

1.6 (±1.1) × 102

0

4.9 (±0.16) × 102

   G248W

9.1 (±10.3) × 106

7.5 (±1.7) × 105

32

2.8 (±0.11) × 104

   G345R

3.7 (±0.90) × 104

1.3 (±)0.3 × 102

0

4.1 (±0.25) × 102

   I257M

1.8 (±0.009) × 107

4.6 (±0.47) × 106

64

8.3 (±0.29) × 104

   K286E

1.4 (±0.02) × 107

1.5 (±0.27) × 106

32

7.9 (±0.38) × 104

   N234Y/I257M

4.1 (±1.9) × 103

3.5 (±4.7) × 102

0

1.6 (±0.24) × 104

   N234Y/K286E

1.6 (±0.11) × 105

1.1 (±0.22) × 104

0

2.1 (±0.32) × 104

   I257M/K286E

9.8 (±0.29) × 103

1.2 (±0.17) × 104

0

1.0 (±0.10) × 104

   N234Y/I257M/K286E

4.3 (±0.49) × 106

4.7 (±0.56) × 106

8

7.0 (±0.37) × 104

NAA*

6.9 (±1.4) × 105

6.7 (±1.5) × 105

2

1.7 (±0.26) × 103

No NA

1.3 (±0.9) × 103

1.3 (±0.7) × 102

0

4.3 (±0.1) × 102

NAA

1.0 (±0.57) × 104

4.9 (±3.4) × 102

0

6.0 (±0.27) × 102

   V119E

5.4 (±0.6) × 103

7.0 (±6.6) × 102

0

6.7 (±0.31) × 102

   Y234N

1.3 (±0.2) × 104

7.3 (±4.9) × 102

0

4.2 (±0.27) × 102

   R241M

1.2 (±0.2) × 104

9.7 (±5.1) × 102

0

4.4 (±0.12) × 102

   R345G

1.3 (±0.1) × 104

7.8 (±2.2) × 102

0

4.3 (±0.42) × 102

   V119E/Y234N

9.1 (±2.9) × 103

6.9 (±3.9) × 102

0

4.6 (±0.14) × 102

   V119E/R241M

1.1 (±0.3) × 104

5.7 (±1.3) × 102

0

4.0 (±0.04) × 102

   V119E/R345G

1.2 (±0.1) × 104

5.0 (±0.6) × 102

0

4.2 (±0.16) × 102

   Y234N/R241M

7.5 (±3.0) × 103

1.5 (±1.0) × 103

0

4.2 (±0.16) × 102

   Y234N/R345G

8.2 (±2.8) × 103

1.1 (±0.9) × 103

0

4.3 (±0.10) × 102

   R241M/R345G

1.8 (±0.2) × 104

1.7 (±0.8) × 103

0

8.2 (±0.47) × 102

V119E/Y234N/R241M

1.0 (±0.05) × 104

2.8 (±1.2) × 102

0

4.2 (±0.23) × 102

   V119E/Y234N/R345G

1.0 (±0.3) × 104

3.3 (±1.8) × 102

0

4.4 (±0.29) × 102

V119E/R241M/R345G

2.1 (±0.25) × 105

1.1 (±0.1) × 104

0

5.4 (±0.09) × 102

Y234N/R241M/R345G

ND

ND

ND

ND

V119E/Y234N/R241M/R345G

2.5 (±0.6) × 106

1.5 (±0.1) × 106

2

2.7 (±0.99) × 103

NAC (NAA 176-230)

1.6 (±0.05) × 107

3.0 (±0.57) × 106

64

5.5 (±0.08) × 104

NAC (NAA 88-230)

5.0 (±0.50) × 105

1.9 (±0.58) × 104

0

2.3 (±0.07) × 104

NAC (NAA 1-230)

1.6 (±0.39) × 105

6.8 (±2.1) × 103

0

4.5 (±0.10) × 103

   S95R

4.3 (±0.07) × 105

1.9 (±0.5) × 104

0

2.1 (±0.12) × 104

   V99I/H100Y

5.9 (±0.6) × 105

2.6 (±0.1) × 104

0

3.1 (±0.03) × 104

   V119E

1.4 (±0.08) × 107

2.1 (±0.1) × 106

16

3.0 (±0.09) × 104

   N146S

1.5 (±0.2) × 105

7.0 (±1.5) × 103

0

1.1 (±0.02) × 104

   H155Y/T157A

3.8 (±0.8) × 105

2.4 (±0.3) × 104

0

2.0 (±0.06) × 104

NAC (NAA 226-470)

1.3 (±0.35) × 104

1.1 (±0.5) × 103

0

4.0 (±0.09) × 102

NAC (NAA 226-362)

1.1 (±0.50) × 104

1.4 (±0.5) × 104

0

4.3 (±0.02) × 102

NAC (NAA 226-301)

1.2 (±0.09) × 105

2.5 (±0.7) × 103

0

1.3 (±0.02) × 103

   Y234N

7.2 (±0.56) × 106

3.6 (±0.3) × 105

32

1.5 (±0.04) × 104

N247D/W248G/Q249L

1.0 (±0.31) × 106

6.2 (±2.6) × 103

0

1.5 (±0.09) × 103

   M257I

7.1 (±1.37) × 106

6.0 (±1.0) × 104

4

1.2 (±0.01) × 104

   V263T/V266I

6.5 (±0.80) × 104

1.1 (±0.6) × 103

0

4.8 (±0.37) × 102

   E269N

2.5 (±0.52) × 105

6.4 (±3.0) × 103

0

1.1 (±0.07) × 103

   Y273S

1.2 (±0.07) × 105

2.9 (±0.6) × 103

0

5.0 (±0.26) × 102

   A284T

1.0 (±0.08) × 105

2.6 (±1.0) × 103

0

6.2 (±0.15) × 102

   E286K

1.8 (±0.004) × 107

2.2 (±0.1) × 106

Und

1.6 (±0.04) × 104

   I287V

6.1 (±0.45) × 105

1.2 (±0.1) × 104

0

1.2 (±0.01) × 103

   T288M

1.6 (±0.06) × 106

6.0 (±0.3) × 104

0

5.2 (±0.18) × 103

  1. α All values are in relative light units (RLU). Standard errors of the means are in parentheses.
  2. ND, not determined
  3. Und, undiluted
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Virology Journal

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