Name | Mutation site | Glycosylation |
---|
 | prM-15 | E-154 | prM | E |
---|
pVAX-prME-wt | Asn Asn Thr | Asn Tyr Ser | + | + |
pVAX-prME-M1 | Ala ÆŸ Asn Thr | Asn Tyr Ser | - | + |
pVAX-prME-M2 | Asn Asn Thr | Ala ÆŸ Tyr Ser | + | - |
pVAX-prME-M3 | Ala ÆŸ Asn Thr | Ala ÆŸ Tyr Ser | - | - |
- Amino acid residues of JEV prME protein targeted for mutagenesis. prME -M1 and prME -M2 are single site mutants, whereas prME -M3 is the double mutant. In all mutants, the Asn codon was replaced by an Ala codon in its N-glycosylation sites (N-X-T/S). Asterisks represent mutated N-glycosylation sites.