Figure 2From: Mutation of putative N-Linked Glycosylation Sites in Japanese encephalitis Virus Premembrane and Envelope proteins enhances humoral immunity in BALB/C mice after DNA vaccinationWestern blotting analysis of the expression of recombinant plasmids and β-actin. (A)Samples were harvested from cell lysates at 48 h after transfection. prME-WT, prME-M1, PVAX-prME-M2, and PVAX-prME-M3 proteins were incubated with PNGase F (+) or buffer only (−). (B) β-actin was used as internal control.Back to article page