Biological and immunological characterization of recombinant Yellow Fever 17D Viruses expressing a Trypanosoma cruzi Amastigote Surface Protein-2 CD8+T cell epitope at two distinct regions of the genome

Table 2 Immunogenicity of recombinant YF 17D viruses in A/J mice

Immunogen ^a	Animals (n)	PRNT ₅₀ ^a		Protection after YF17D challenge (%)
		1 dose	2 doses
YF 17DD	15	358 ± 119	1007 ± 243	100
YF17D/E200/Tc	15	41 ± 12*	124 ± 67**	NT^b
YF17D/NS2B3/Tc	15	90 ± 20*	274 ± 240**	100
199 Earle's Medium	15	< 10	< 10	-

^aReciprocal of the dilution yielding 50% plaque reduction due to neutralizing antibodies in the serum analyzed. Values represent mean of three independent experiments (pooled serum) ± standard deviation (SD). * first and **second dose titers displaying statistically significant differences related to YF17DD group (P < 0.05, Tukey's test)
^b NT; Not tested

ISSN: 1743-422X