Table 1 Primers used for amplification of the Flury LEP strain
From: Molecular characterization of a Chinese variant of the Flury-LEP strain
Designation | Sequence of primers | Length | Location |
|---|---|---|---|
RLM-3' RACE oligonucleotidesa | 5'-GTCGTACTAGTCGACGCGTGGCCTAG-3' | 26 | Â |
3' RACE complementary oligonucleotidesa | 5'-GGCCACGCGTCGACTAGTAC-3' | 20 | Â |
LEPR1(antisense) | 5'-CAAGAGGGCCCCTGGAATCA-3' | 20 | 2872-2892 |
LEPF2(sense) | 5'-TCCAGGGGCCCTCTTGAAGGGGAG-3' | 24 | 2875-2899 |
LEPR2(antisense) | 5'-ATGACCGGTCTTCACAGTCTGGTC-3' | 24 | 4881-4905 |
LEPF3(sense) | 5'-GTGAAGGCCGGTCATCCTTTTGACAATT-3' | 28 | 4888-4916 |
LEPR3(antisense) | 5'-CAAGAGACTCGGGCCCAT-3' | 18 | 7836-7854 |
LEPF4(sense) | 5'-GATGGGCCCGAGTCTCTTGC-3' | 20 | 7833-7853 |
LEPR4(antisense) | 5'-TAACACAAGATCGATCTGTTG-3' | 21 | 9905-9926 |
LEPF5(sense) | 5'-CCACTATGAAAGAAGGCAACAGATCGATC-3' | 29 | 9888-9917 |
5' RACE Outer Primer | 5'-CATGGCTACATGCTGACAGCCTA-3' | 23 | Â |
5' RACE Inner Primer | 5'-CGCGGATCCACAGCCTACTGATGATCAGTCGATG-3' | 34 | Â |