Table 6 Primer sets for amplification of the LACV RNA
Genome Segment | Primer | Primer Sequence (5'-3') | Primer position | Product Size (bp) |
|---|---|---|---|---|
Primers used for the amplification of genome segments | ||||
S-A | SF1 | AGTAGTGTACCCCACTTGAATAC | 1-23 | 525 |
| Â | SR | CTTAAGGCCTTCTTCAGGTATTGAG | 549-572 | Â |
S-B | SF3 | CTTAAGGCCTTCTTCAGGTATTGAG | 453-476 | 486 |
| Â | SR1 | AGTAGTGTGCCCCACTGAATAC | 963-984 | Â |
M-C | MF1 | AGTAGTGTACTACCAAGTATAGATGAACG | 1-27 | 2211 |
| Â | MR10 | GACTCCTTTCCTCTAGCAAGG | 2239-2258 | Â |
M-D | MF9 | CAGACAACATGGAGAGTGTAC | 1798-1818 | 1786 |
| Â | MR5 | GTCAAATCTGGGAACTCCATTGCC | 3605-3628 | Â |
M-E | MF15 | CAAGCTCATGGGGATGCGAAGAG | 3249-3271 | 1235 |
| Â | MR1 | AGTAGTGTGCTACCAAGTATA | 4507-4527 | Â |
L-F | LF1 | AGTAGTGTACCCCTATCTACAAAAC | 1-25 | 2955 |
| Â | LR20 | GTTTTCCCTCTGTTCGCACTC | 2381-2401 | Â |
L-G | LF8A | CAACTTGCCTACTATTCAAAC | 1899-1919 | 2164 |
| Â | LR7 | CCAATCCAACTGTACTAATCATTGAC | 4084-4108 | Â |
L-H | LF8 | GCTACCAGGGCAGTCAAATGACCC | 3987-4011 | 1949 |
| Â | LR10 | CCTCTGCAACGTTAACTACACATACTG | 5961-5986 | Â |
L-I | LF10 | CAGATATTGTCTGGTGGCCATAAAGCC | 5288-5314 | 1646 |
| Â | LR12 | AGTAGTGTGCCCCTATCTTC | 6961-6980 | Â |
Primers used for the amplification of NSs and NSm | ||||
S | NSsF-int | TTTGAAAATAAATTGTTGTTGACTG | 24-48 | 472 |
| Â | NSsR-int | CCCACTGTCCCATCCTACAC | 476-495 | Â |
S | NSsF-ext | ACTCCACTTGAATACTTTGAAAATAAA | 9-35 | 563 |
| Â | NSsR-ext | CAATGGTCAGCGGGTAGAAT | 552-571 | Â |
M | NSmF-int | CTGGATTGTGCCCTGGTTAT | 918-937 | 598 |
| Â | NSmR-int | TCAGTCTCTAGGCAGGTGGTG | 1495-1515 | Â |
M | NSmF-ext | GCGGTGCTCGCTATGATACT | 870-889 | 742 |
| Â | NSmR-ext | AATTGGGTTGCAATGTTGGT | 1592-1611 | Â |