Figure 2
From: Species-independent bioassay for sensitive quantification of antiviral type I interferons
Measurement of IFN in samples by the RVFV-Ren antiviral assay. (A) Generation of the standard curve. All data points from Figure 1B which are above the sensitivity threshold and within the linear range of the assay (from 1-50 U/ml) are plotted. Non-linear regression analysis was performed to generate a standard curve and to compute the regression equation. Values for the slope (k) and the y-axis interception point (d) were -0.3988 and 5.159, respectively. The coefficient of regression (R2) indicates the linearity of detection. (B) Analysis of two samples with low and high concentration of IFN. Supernatants from human A549 cells infected with wt mutant La Crosse virus were first sterilized with β-propiolactone (see Methods section) and then analyzed for inhibition of RVFV-Ren. Sterilized supernatant from uninfected cells served as control (mock). The IFN content of all samples was extrapolated from the standard curve shown in (A). All measurements were performed in triplicate; columns show means ± SD, with actual values given above each column.