Figure 5
The effect of CSFV NS2 expression on porcine cyclin A and GRP78 transcription in cultured SUVEC cells. Total RNA was extracted from cells expressing either GFP alone, GFP-NS2 fusion, NS2-GFP fusion or untransfected cells. Real-time RT-PCR analysis of (A) cyclin A and (B) GRP78 mRNA levels were normalized to the corresponding CT value for porcine β-actin mRNA. The basal expression level in untransfected controls was assigned a value of 1 for each experiment.