Figure 2From: Molecular characterization of two distinct monopartite begomoviruses infecting tomato in indiaDetection of viral genome in infected tomato leaf samples and the ORFs encoded by Tomato leaf curl virus. (A) Rolling Circle Amplification (RCA) of total DNA for ToLCV-K3/K5 samples using Ø29 polymerase digested by Bam H I (lanes 1, 2), Xba I (lanes 3, 4) and Xho I (lanes 5, 6). (B) RCA of total DNA for ToLCV-CTM digested by Bam H I, EcoR I, Sma I, Nco I, Xmn I and Nde I (lanes 1-6 respectively). M denotes size marker lane. (C) Genome map of Tomato leaf curl virus DNA A showing the sense (AV1and AV2) and complementary strand ORFs (AC1, AC2, AC3, AC4).Back to article page