Skip to main content

Table 3 Specificity of the BKV Real-Time Quantitative PCR Assay

From: Development of a real-time quantitative PCR assay for detection of a stable genomic region of BK virus

   

Crossing Point

   

BKV_5.1/Polyoma_4.2

Copies BKV/Reaction*

Copies JCV/Reaction*

# of Reactions

Mean

Std Dev

CV (%)

1.5 × 107

7.0 × 103

10

18.43

0.07

0.37

1.5 × 106

7.0 × 103

10

21.99

0.02

0.11

1.5 × 105

7.0 × 103

10

25.61

0.03

0.10

1.5 × 104

7.0 × 103

10

29.14

0.04

0.15

1.5 × 103

7.0 × 103

10

33.06

0.08

0.25

1.5 × 102

7.0 × 103

10

36.74

0.66

1.80

1.5 × 101

7.0 × 103

10

39.61

1.22

3.08

1.5 × 107

0

2

18.41

0.02

0.12

1.5 × 106

0

2

22.01

0.08

0.35

1.5 × 105

0

2

25.69

0.01

0.06

1.5 × 104

0

2

29.28

0.08

0.27

1.5 × 103

0

2

33.03

0.05

0.15

1.5 × 102

0

2

36.91

0.27

0.73

1.5 × 101

0

2

39.94

1.97

4.94

0

1.4 × 104

10

---

---

---

Negative Control

 

5

---

---

---