Measurement of Epstein-Barr virus DNA load using a novel quantification standard containing two EBV DNA targets and SYBR Green I dye

Table 4 EBV DNA load and antibody titre detection rates in the population samples (Group 5, n = 218).

Target	Positive n (%)	Detectable Range	Spearman correlation (p)
			EBNA-1 DNA load	BHRF-1 DNA load	Combined EBV Targets DNA load	VCA IgG
EBV EBNA-1 DNA load (copies/ml)	24 (11.0%)	2.0 × 10² - 9.1 × 10⁴	1.00
EBV BHRF-1 DNA load (copies/ml)	47 (21.6%)	2.0 × 10² - 3.3 × 10⁴	0.63 p < 0.001	1.00
Combined EBV targets DNA load (mean of BHRF & EBNA loads where both positive) (copies/ml)	49 (22.5%)	2.0 × 10² - 6.2 × 10⁴	0.73 p < 0.001	0.97 p < 0.001	1.00
Viral capsid antigen IgG (titres)	209 (95.9%)	1:10 - 1:5120	0.11 p = 0.11	0.13 p = 0.05	0.14 p = 0.04	1.00

Abbreviations: EBV, Epstein-barr virus; EBNA-1, Epstein-barr virus nuclear antigen-1; BHRF-1, BamHI fragment H rightward open reading frame-1; VCA, viral capsid antigen; IgG, immunoglobulin G; Pos, positive

ISSN: 1743-422X