Figure 5From: Serological characterization of guinea pigs infected with H3N2 human influenza or immunized with hemagglutinin proteinPepScan ELISA of serum pools from guinea pigs immunized with recombinant HA protein. Group serum pools (1:750 dilution) were analyzed for recognition of linear epitopes by reactivity to overlapping peptides bound to microtiter plates. The black bars indicate the magnitude of the ELISA reactivity as a measure of Optical Density (O.D.) for the recognition of specific peptides. Sequential bleeds were tested from the prebleed (A) and 3 (B), 5 (C), and 12 (D) weeks after the initial immunization. Reactivity to the peptides was compared between the three groups to identify potential linear epitopes. Group 1: mock immunized negative control group (left column), Group 3: lower dose HA-immunized (center), Group 4: higher dose HA-immunized (right column).Back to article page