Detection and quantitation of HPV in genital and oral tissues and fluids by real time PCR

Table 1 Sequence of HPV type-specific L1 primers and probes used for qPCR.

Oligonucleotide	Sequence (5'→3')	Concentration/Rx
HPV6L1F	TGGGGTAATCAACTGTTTGTTACTGTGGTA	400 nM
HPV6L1R	GCATGTACTCTTTATAATCAGAATTGGTGTATGTG	400 nM
HPV6L1probe	Cy5- GACATTATGTGCATCCGTAACTAC-BHQ2	200 nM
HPV11L1F	CTGGGGAAACCACTTGTTTGTTACTGTG	400 nM
HPV11L1R	CGCATGTATTCCTTATAATCTGAATTAGTGTATGTA	400 nM
HPV11L1probe	TexasRed- GACACTATGTGCATCTGTGTCTAA-BHQ1	800 nM
HPV16L1F	TTGTTGGGGTAACCAACTATTTGTTACTGTT	400 nM
HPV16L1R	CCTCCCCATGTCTGAGGTACTCCTTAAAG	400 nM
HPV16L1probe	6FAM- GTCATTATGTGCTGCCATATCTACTTC-TAMRA	400 nM
HP18L1F	GCATAATCAATTATTTGTTACTGTGGTAGATACCACT	400 nM
HP18L1R	GCTATACTGCTTAAATTTGGTAGCATCATATTGC	400 nM
HPV18L1probe	HEX- AACAATATGTGCTTCTACACAGTCTCCTGT-BHQ2	100 nM
HPVE1F1	ANANGCTGTGCAKGNNCTAAAACGAAG	300 nM
HPVE1R1	AGTTTCCACTTCAGTATTGCCATA	300 nM
HAPBF	TGAAGGTGGAGGACATTCCTCTA	400 nM
HAPBR	CTGGAATTGCGATTTCTGGTAA	400 nM
HAPBprobe	Cyan500- CGAGAATCACCCTGCCAGACTTCCGT-BBQ	100 nM

ISSN: 1743-422X