Table 6 Real time PCR assays for VHS virus, Paranucleospora theridion, Flavobacterium psychrophilum, Candidatus Piscichlamydia salmonis and a new species of chlamydia from gills of Atlantic salmon.
Target | Code: | Primers/probe | Amplicon size | Reference |
|---|---|---|---|---|
VHS virus | VHSV F08 | TGT CCG TKC TTC TCT CCT ATG TAC T | Â | Modified |
| Â | VHSV probe | CTC ACA GAC ATG GG | 109 nt | [44] |
| Â | VHSV R08 | GCC CTG RCT GMC TGT GTC A | Â | Modified |
Paranucleospora | PT-F | CGG ACA GGG AGC ATG GTA TAG | Â | Â |
theridion | PT-probe | TTG GCG AAG AAT GAA A | 59 nt | [49] |
| Â | PT-R | GGT CCA GGT TGG GTC TTG AG | Â | Â |
Flavobacterium | Flavo-R | TGT AAA CTG CTT TTG CAC AGG AA | Â | Â |
psychrophilum | Flavo-probe | AAA CAC TCG GTC GTG ACC | 72 nt | Present study |
| Â | Flavo-F | GAT CCT TAT TCT CAC AGT ACC GTC AA | Â | Â |
Candidatus | Pch-F | TCA CCC CCA GGC TGC TT | Â | Â |
Piscichlamydia | Pch-probe | CAA AAC TGC TAG ACT AGA GT | 60 nt | Present study |
salmonis | Pch-R | GAA TTC CAT TTC CCC CTC TTG | Â | Â |
New species of | Sch-F | GGG TAG CCC GAT ATC TTCA AAG T | Â | Â |
gill chlamydia | Sch-probe | TCC TTC GGG ACC TTA C | 66 nt | Present study |
| Â | Sch-R | CCC ATG AGC CGC TCT CTC T | Â | Â |
Elongation factor | EL1A-elaf | CCC CTC CAG GAC GTT TAC AAA | Â | Â |
1 alpha | EL1A-elam1 | ATC GGT GGT ATT GGA AC | 57 nt | [45] |
S. salar | EL1A-elar | CAC ACG GCC CAC AGG TAC A | Â | Â |