Figure 4
Detection of LAMP products by observing white turbidity and color of the reaction mixture. (A) shows white turbidity of the reaction mixture by magnesium pyrophosphate; (B) shows color (green) of the reaction mixture after addition of SYBR Green I. N, negative control; 1--12, reaction carried out using 10-fold serial dilutions of plasmid (pVP3) DNA (2.8 × 1011 copies/μl). 1: 2.8 × 100, 2: 2.8 × 101, 3: 2.8 × 102, 4: 2.8 × 103, 5: 2.8 × 104, 6: 2.8 × 105, 7; 2.8 × 106, 8: 2.8 × 107, 9: 2.8 × 108, 10: 2.8 × 109, 11: 2.8 × 1010, 12: 2.8 × 1011 copies/μl, respectively; 13:+, positive control.