Figure 3

Transduction of rat islets with pseudotyped AAV vectors at high AAV MOIs and a low Ad5 MOI. Rat islets were transduced with AAV2-nls-eGFP packaged into the capsids of AAV2 (A, B, J, K), AAV5 (C, D), AAV8 (E, F) or BAAV (H, I), at an AAV MOI of 10,000 (A-G) or 30,000 (H-L) and an Ad5 MOI of 1 (A-L). The islets were visualized by confocal microscopy on day 5 after transduction. (A, C, E, H, J) Fluorescent. (B, D, F, I, K) DIC. On day 6, flow cytometry analysis was performed on dispersed islet cells (G, L), including negative controls infected with Ad5 only. AAV vectors used in panels A-G were purified on iodixanol gradients. AAV vectors used in panels H-L were purified on cesium chloride gradients.