Figure 5

Co-localization of p37-GFP with p230 and CI-MPR in infected and uninfected cells in the presence and absence of ST-246. Cell monolayers were grown on chamber slides and (A) infected with 8 PFU/cell of vvF13LGFP or (B) transfected with 1 μg of plasmid expressing p37-GFP in the presence or absence of 10 μM ST-246. At 14–16 hpi cells were fixed in 4% paraformaldehyde, permeablized with 0.2% TritonX-100 and stained for 1 hour with anti-p230 antibody or anti-CI-MPR antibody. Proteins were visualized using Alexa 594-conjugated secondary antibody. Samples were mounted in ProLong Gold Antifade Reagent (Invitrogen Molecular Probes) containing DAPI for nuclear staining and analyzed using a Zeiss LSM 510 confocal laser-scanning microscope. Images are representative of 2 separate experiments in which a minimum of 50 infected cells displaying a similar fluorescent phenotype were observed. Images were collected and processed using LSM 510 acquisition and Adobe Photoshop software. Bar, 5 μm.