Figure 2
From: Identification of a novel betaherpesvirus in Mus musculus
The novel cytomegalovirus of Mus musculus : Multiple sequence alignment and phylogenetic analysis. (a) The MmusCMV-2 DPOL sequence amplified by panherpes consensus PCR (178 bp) was translated into a 59 aa sequence, and a multiple sequence alignment with the corresponding sequences of MCMV, RCMV-M, RCMV-E, HCMV and HHV-6b was generated using the ClustalW module of MacVector™ 10.6. Identical and similar amino acids are boxed, the last in inverse type. Mismatches are given in lower type. (b) A phylogenetic tree was constructed using the nucleic acid sequences encoded by the gB-DPOL segments of MmusCMV-2 and those of known human, mouse and rat cytomegaloviruses (GenBank accession numbers in the Findings section). Abbreviations of common names are used, and those of species names according to the ICTV (International Committee on the Taxonomy of Viruses) are given in parentheses. A multiple alignment of 3.4 kb was analysed with the neighbour-joining method. A rooted phylogram is shown, with HHV-6a as outgroup. The branch length is proportional to evolutionary distance (scale bar). Results of bootstrap analysis (1000 replicates) are indicated at the nodes of the tree. The novel MmusCMV-2 is highlighted in bold type.