Figure 1
Attachment studies using a clinical isolate of CVB5. A) 3 × 104dpm (corresponding to MOI 0.05 TCID50/cell) was incubated with 2.5 × 105 cells in suspension at 4°C for 2 h. Attachment was measured using scintillation counting. B) MOI 0.05 TCID50/cell of the labeled, purified virus and C) MOI 0.05 TCID50/cell of unpurified virus incubated as described in A) and the amount of virus bound to the cell surface was measured with a two-step RT-PCR. Results are presented as mean ± SEM.