Figure 2
Annotated amino acid sequence of scFv CUF37-2a. Nucleotide sequences were edited and translated using Lasergene software http://www.dnastar.com. The Pel B leader peptide to direct secretion of the scFv to the periplasm of E. coli host cells during heterologous expression is underscored with a dotted line. The cleavage point of this signal peptide is indicated with a block arrow. The presence of a Flag-tag antibody at the N-terminus of the protein is shown with a double underline. The poly-glycine linker joining the VL and VH chains of the scFv is underlined with a single solid line. The Framework Regions (FR) and Complementarity Determining Regions (CDR) within the scFv sequence are indicated with arrows and with shading respectively.