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Figure 1 | Virology Journal

Figure 1

From: The ORF59 DNA polymerase processivity factor homologs of Old World primate RV2 rhadinoviruses are highly conserved nuclear antigens expressed in differentiated epithelium in infected macaques

Figure 1

Comparison of the ORF59 homologs of Old World primate RV1 and RV2 rhadinoviruses. The orf59 genes from the RV1 rhadinoviruses from chimpanzee (PtrRV1) and three species of macaque, M. mulatta (RFHVMm), M. nemestrina (RFHVMn) and M. fascicularis (RFHVMf), and the ORF59 genes from the RV2 rhadinoviruses from chimpanzee (PtrRV2) and the two species of macaque, M. nemestrina (MneRV2) and M. fascicularis (MfaRV2), were cloned using a CODEHOP PCR approach (see Materials and Methods) and the encoded amino acid sequences were aligned with the previously published sequences of the human RV1 rhadinovirus, KSHV (NP_572115) and the rhesus macaque RV2 prototype, RRV17577 (AAD21393). Identical residues in six of the nine sequences are highlighted in black. Identical residues in at least three of the four RV2 sequences are highlighted in blue, whereas identical residues in at least three of the RV1 sequences are highlighted in purple. Domains involved in nuclear localization (NLS), dimer formation, DNA-binding and DNA polymerase (Pol-8) binding [6, 11, 21] of KSHV ORF59 are indicated. The RV2-specific antigens of RRV and MneRV2 ORF59 used to produce the anti-RV2 ORF59 rabbit polyclonal antisera are underlined (see Materials and Methods).

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Virology Journal

ISSN: 1743-422X