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Figure 5 | Virology Journal

Figure 5

From: An HPV 16 L1-based chimeric human papilloma virus-like particles containing a string of epitopes produced in plants is able to elicit humoral and cytotoxic T-cell activity in mice

Figure 5

Determination of the antibodies response of mice to VLPs by ELISA and hemmaglutination inhibition assay. Groups of C57BL/6 mice were immunized with Gardasil® and with purified VLPs and cVLPs derived from tomato fruits. Serum was collected from each mice and used for: A, determination of antibodies against different types of VLPs by ELISA. Bars, mean and SE (n = 6). Sera dilution 1:100. B, hemagglutination inhibition assay: sera from C57BL/6 mice (n = 6 in all cases) immunized with Gardasil®, VLPs and cVLPs and the pre-immune group were assayed at 1:1600, 1:800, 1:400, 1:200 and 1:100 dilutions as described in the text. (-), negative control (erythrocytes in the absence of VLPs), 100 ng of VLPs (a), cVLPs (b), and Gardasil® (c). VLPs incubated with erythrocytes were used as positive controls for the hemagglutination assay.

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Virology Journal

ISSN: 1743-422X