Figure 3From: Bovine viral diarrhea virus NS4B protein is an integral membrane protein associated with Golgi markers and rearranged host membranesA. Localization of BVDV NS4B in infected MDBK cells. Cells were grown on coverslips and infected with BVDV at MOI of 10. At 12 h (i and ii), 18 h (iii and iv) and 24 h.p.i (v and vi), cells were processed for immunofluorescence (IF) with NS4B-specific antibody (1/50 dilution) and Alexa fluor 488-conjugated secondary antibody (1/500). Nuclei were stained with DAPI. Notice the Golgi-like NS4B distribution at 12 h and 18 h.p.i., whereas foci are seen at 24 h.p.i No fluorescence is displayed in mock-infected cells (vii). Bars = 10 μm. B. BVDV NS4B partially colocalizes with Golgi markers. Cells were grown on coverslips and infected with BVDV as above. At 18 h.p.i., cells were processed for IF with NS4B- (ii and iv; vi and viii), TGN38- (iii and iv) and Golgin97 (vii and viii)- specific antibodies. Notice the colocalization of NS4B with TGN38 or Golgin97 protein. Mock-infected cells, stained with anti TGN38 (i) or Golgin97 (v), are also shown.Back to article page