Figure 2

Inconsistent challenge results from repeated stable transductions of 6.3. (A) We challenged G418 selected CEMT4 polyclonal populations of each of our 6 single shRNA vectors with HIV-1. Suppressive activities were inferred by intracellular p24 levels measured between 5 and 8 days later. Each population was assayed in 3 independently repeated experiments. A control vector expressing a single shRNA unmatched to HIV-1 was also tested 3 times (grey points), with the average values of 3 experiments and 95% confidence intervals (CI) shown. (B) Five separate 6.3 polyclonal populations were generated through independent t ransductions (t1 to t5) using 3 different lentiv iral batches (v1, 2, and 3). Each population was similarly selected and challenged in 3 independently repeated experiments with HIV-1. The control vector was a combination of 6 shRNAs unmatched to HIV-1 that were assembled in the same format as 6.3 (grey points), with the average values of 3 experiments and 95% confidence intervals (CI) shown.