Figure 1

Phenotypic characterisation of Ad2-BAC46 and Ad2-ts1. A) Diagnostic DNA sequencing around nucleotide 22187 (P137L codon of the protease L3/p23) from purified Ad2 (Ad2-BAC53), Ad2-ts1, a derivate from Ad2-ND1 related to "adenoid 6" (ATCC # V-846), Ad2-BAC46 and the reverted Ad2-BAC46_r genomes. Codon 137 of Ad2 consisted of CCC encoding proline (P), Ad2-ts1 and Ad2-BAC46 of CTC (leucine, L), and Ad2-BAC46_r CCA (proline). B) SDS-12%-PAGE and Coomassie-blue analyses of purified Ad2 wild type (wt), Ad2-ts1 (grown at 40°C), Ad2-BAC46 (grown at 32°C), Ad2-BAC46 (grown at 40°C) and Ad2-BAC46_r (grown at 40°C) with relative molecular weights on the left side (SDS-PAGE Molecular Weight Standard, Broad Range 161-0317, BioRad), virion proteins (right), and precursor proteins (p). C) Flow cytometry of dextran-FITC in HeLa cells [31] infected at moi 20 with Ad2, Ad2-ts1 (grown at 40°C), or Ad2-BAC46 (grown at 40°C) at 37°C for 15 min. Results show fold-stimulation of dextran uptake over noninfected cells.