Figure 2

Southern hybridization with PAdV-3 DNA. Fractionated Hind III digested wt PAdV-3 genomic DNA was probed with [³²P]-labled cDNAs generated by RT of the RNAs isolated from purified wt PAdV-3 mature capsids, empty/intermediate capsids, wt PAdV-3-infected VIDO R1 cells or uninfected ST cells as described in the text. Blots in c, d, e, and f were exposed for the same time with PhosphorImager screen (Bio-Rad). Presence (+). Absence (-). EtBr, Ethidium-bromide (EtBr) staining of Hind III digested PAdV-3 genomic fragments.1 Kb DNA ladder (M). Mature(Ma);Empty/Intermediate(E-I).