Figure 3

Schematic overview of the experimental strategy to identify host genes involved in HIV-1 infection using RHGP. MT4 cell lines expressing the transactivator R1 were first constructed. Following transduction with the RHGP vector antibiotic selection was used to establish an "RHGP library" of gene perturbations. Next the RHGP library cells were challenged with a lethal infection of HIV-1 virus in the presence of the inducer RSL1. Survivors were cloned and then validated by reversing the RHGP phenotype in the absence of RSL1. The genomic DNA was then isolated from those validated clones and the identity of the target gene, along with the orientation of the GSV integration event ("Sense" or "Antisense") was then determined.