Table 2 Oligonucleotide sequences of the primers and probes used in the GPV FQ-PCR method (Oligonucleotide positions have been determined by referring to the gene sequence of U25749)
Name | Sequence 5' to3' | Position | Amplicon size (bp) |
|---|---|---|---|
GPV-F | GTGCCGATGGAGTGGGTAAT | 3084-3103 | 60 |
GPV-R | ACTGTGTTTCCCATCCATTGG | 3122-3143 | |
GPV-FP | 6FAM-FTCGCAATGCCA ATTTCCCGAGGP--TAMRA | 3098-3120 | |
VP3-1 | AAGCTTTGAAATGGCAGAGGGAGGA | 3008-3033 | 1658 |
VP3-2 | GGATCCCGCCAGGAAGTGCTTTATTTGA | 4637-4665 |