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Figure 1 | Virology Journal

Figure 1

From: A catalytically and genetically optimized β-lactamase-matrix based assay for sensitive, specific, and higher throughput analysis of native henipavirus entry characteristics

Figure 1

Synthesis of the β-lactamase-matrix (βla-M) fusion construct and its incorporation into virus-like particles (VLPs). a) Codon usage comparisons between wild-type NiV-M (henipavirus), βla (bacteria) and average Homo sapiens genes. For clarity, only representative amino acids with significant differences in codon usage frequencies between Homo sapiens and NiV-M or βla genes are shown. Note the skewing towards more rarely used mammalian codons. Overall, codon usage for amino acids not shown cumulatively demonstrate a pattern of rare mammalian codon usage (see Additional file 1). b) Cell lysates from transfected 293T cells were blotted for protein expression using anti-M antibodies. c) VLPs collected from NiV-M+NiV-F/G or βla-M+NiV-F/G transfected 293T cell supernatants were purified as described in the materials and methods. VLPs were lysed and blotted for protein incorporation using anti-NiV-M antibodies along with anti-HA (NiV-G) antibodies to quantify total VLP production.

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Virology Journal

ISSN: 1743-422X