Figure 5
From: 9-aminoacridine Inhibition of HIV-1 Tat Dependent Transcription
p21WAF1 binds to cyclin T and cdk9 in vitro. One μg of GST, GST-p21, GST-p21 (C), or GST-p21 (N) were added to 1 mg of CEM cell lysates and allowed to bind overnight. The next day, complexes were bound to glutathione sepharose beads, washed, and analyzed by SDS-PAGE, followed by western blotting for cyclin T and cdk9 (Panel A) or cdk2 (Panel B). ext = Extract.