Figure 5
From: The role of NH4Cl and cysteine proteases in Human Papillomavirus type 16 infection
Cathepsin B treatment overcomes block of infection caused by furin protease inhibition. 293 cells incubated with 60 μM of furin inhibitor DEC-RVKR-CMK overnight were infected in the presence of mock treated (control), cathepsin B protease (A, 37 pM) or cathepsin L protease (B, 37 pM) treated reporter-virions for various amounts of time. Cells were analyzed 48 hours post binding by flow cytometry for the presence of DsRed expression. Statistics were analyzed by 1 tailed t-test and found to have a significant difference at P < 0.05.