Correlating novel variable and conserved motifs in the Hemagglutinin protein with significant biological functions

Table 2 Amino acid substitutions in the different isolates from 1969–1999 used to extrapolate the genetic distance in the different MEME blocks

YEARS	Number of amino acid substitutions	% CHANGE BETWEEN YEARS	MEME Block	Genetic Distance
1968–1969	5	0.883392	1	0.08943
1969–1971	12	1.943463	2	0.3678
1971–1972	11	1.943463	3	0.228
1972–1973	22	3.886926	4	0.16
1973–1974	5	0.883392	5	0.142
1974–1975	15	2.650177	6	0.293
1975–1980	29	4.946997	7	0.8292
1980–1982	6	1.060071	8	0.048
1982–1983	13	2.296820	9	0.288
1983–1984	2	0.353357	10	0.212
1984–1985	1	0.176678	11	2
1985–1987	7	1.236749	12	0.167
1987–1988	3	0.530035	13	1.833
1988–1989	8	1.423488
1989–1992	10	2.473498
1992–1999	23	4.240283

Using ClustalW alignment the number of observed substitutions for each of the consensus sequence and the equivalent years are tabulated using Infoalign. The highest aminoacid substitution (29 aa substitutions over the entire sequence) was in Years 1980. The genetic distance in each MEME block is calculated showing that MEME blocks 1 and 8 are conserved (bold), MEME blocks 7, 11 and 13 are highly variable and the other MEME blocks show intermediate variability.

ISSN: 1743-422X