Figure 4

Co-localization CXCR4 and HIV-1 glycoprotein in HIV-1 infected H9 cells. Four days after HIV-1 infection, cells were fixed and permeabilized with saponin. Cells were then labeled with a human monoclonal antibody that interact with SU and then rhodamine-conjugated goat anti-human antibodies (Panel C: red fluorescence) and with 12G5 mAb followed by fluorescein-conjugated goat anti-mouse antibodies (Panel D:green fluorescence). Panel A: phase contrast image. Panel B represents a superposition of green and red fluorescence, with costained regions appearing in yellow. Yellow regions in panel B indicate the colocalization of chemokine receptor CXCR4 and HIV-1 proteins.