Figure 2
From: Isolation and characterization of cidofovir resistant vaccinia viruses
Mapping and marker rescue of recombinant viruses. A. Map of PCR fragments in the E9L region that were used for marker rescue mapping experiments and reconstruction of resistant viruses. B. Results of mapping experiment for CDVR 1 and 2. Monolayers of BSC 40 cells infected with virus resulting from infection/transfections of VV WR and the indicated PCR fragments and stained with crystal violet. CDV was present at 150 μM. Only those PCR fragments that contain the mutation conferring resistance to CDV are capable of producing recombinant viruses that were detectable in the plaque assay.