Figure 2

The induction of apoptosis in HPV-transformed and non-HPV-transformed cells. (A) HPV-transformed HeLa cells growing on coverslips were transiently co-transfected with plasmids expressing the E2 proteins shown in the figure and a plasmid expressing GFP. After 30 hours the cells were fixed and stained and the number of apoptotic cells in the transfected (green) population determined by counting. The data represent the mean and standard deviation of four independent experiments. (B) The experiment described above was repeated in non-HPV-transformed SAOS-2 cells. In this case 200 ng of the p53 expressing plasmid pCB6-p53 was included in each co-transfection. The data shown is the mean and standard deviation of four independent experiments. (C) The graph shows the luciferase activity found in SAOS-2 cell extracts 24 hrs after transient co-transfection with an E2-responsive reporter plasmid and plasmids expressing the E2 proteins described in Figure 1B. Promoter activity was normalized with respect to transfection efficiency and is shown as fold activation over the reporter alone. The results are the average and standard deviation of four experiments.