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Figure 5 | Virology Journal

Figure 5

From: Dissecting the role of putative CD81 binding regions of E2 in mediating HCV entry: Putative CD81 binding region 1 is not involved in CD81 binding

Figure 5

Determining association of E1E2 mutants. 293T cells were transfected with HCV E1E2 wt, mutant, or E1 alone glycoprotein expression plasmids. Cells were lysed and cleared cell lysate was incubated with anti (α)-E2 antibody. Immune complexes were separated by SDS-PAGE and analyzed by Western Blot for E1 to determine if the E2 and E1 glycoproteins had formed dimers. Image is a composite.

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