Figure 2From: The first hydrophobic region of the HPV16 E5 protein determines protein cellular location and facilitates anchorage-independent growthExpression of the codon-adapted version of HPV16 E5α and corresponding deletions. HEK- 293T cells were transfected with the pFlag-pCMV recombinants and 24 hours thereafter SDS extracts were prepared. Proteins were separated by acrylamide gel electrophoresis and blotted with antibodiesagainst the Flag-epitope.Back to article page