Figure 9

A Single Dose of Recombinant Adenovirus Significantly Suppresses Pregnane × Receptor (PXR) mRNA in Male Sprague Dawley Rats 24 Hours After Treatment. Mean relative intensities of RT-PCR products of PXR (Panels A-D) and the retinoid × receptor (RXR, Panels E-H) after treatment with either WT, AdlacZ, PEGAd, HDAd, or UVAd. mRNA levels are reported as band densities of gene-specific RT-PCR products with respect to the density of products from an internal control (18S rRNA). For all panels, animals dosed with phosphate buffered saline served as controls (Vehicle). Values are presented as the mean ± standard error of 4 animals/treatment/timepoint. Statistical significance was determined between individual treatment groups and vehicle controls by one-way analysis of variance with a Bonferroni/Dunn post-hoc test. *p ≤ 0.05, **p ≤ 0.01.