Figure 1

Administration of a Single Dose of Active Adenovirus Significantly Suppresses Hepatic CYP3A1/2 Protein Expression for 14 Days without Recovery. Immunoblot analysis and representative immunoblots of hepatic CYP3A1/2 protein expression 0.25 (A), 1 (B), 4 (C), and 14 (D) days after a single intravenous dose of wild type adenovirus serotype 5 (WT), a first generation recombinant adenovirus serotype 5 expressing E. coli beta-galactosidase (AdlacZ), PEGylated AdlacZ, (PEGAd), helper-dependent adenovirus serotype 5 expressing beta-galactosidase (HDAd), or inactivated AdlacZ (UVAd) in male Sprague-Dawley rats. Protein levels are reported as the relative density of positive bands with respect to that of a known protein standard in arbitrary units. Values are presented as the mean ± standard error of 4 animals/treatment/timepoint. Statistical significance was determined between individual treatment groups and vehicle controls by one-way analysis of variance with a Bonferroni/Dunn post-hoc analysis. *p ≤ 0.05, **p ≤ 0.01.