Figure 3
From: Iota-Carrageenan is a potent inhibitor of rhinovirus infection
Iota-Carrageenan dose-dependently inhibits HRV2 replication in cell culture. (A) Preincubation of virus with polymer. HeLa cells grown in 12-well plates were infected with HRV2 (0,1 TCID50/cell) in the presence of Iota-Carrageenan at the concentration indicated at the x-axis. 30 minutes after infection the inoculum was removed and medium containing Iota-Carrageenan with the concentration indicated was added. Untreated cells were used as control (mock treated). B. Treatment with polymer after infection. HeLa cells grown in 24-well plates were infected with HRV2 (0,1 TCID50/cell). 30 minutes after infection the inoculum was removed and medium containing Iota-Carrageenan with the concentration indicated at the x-axis was added. Untreated cells were used as control (mock treated). Viral titers in the supernatants of infected cells were determined after 48 h by TCID50 assay on HeLa cells. Values are the means from six parallel titrations, standard deviation is indicated.