Figure 2

Iota-Carrageenan induced inhibition of HRV2 infected cells is dependent on the amount of virus. A. Preincubation of virus with polymer. HeLa cells grown in 96-well plates were infected with HRV2 in the presence of Iota-Carrageenan at concentrations as indicated at the x-axis. B. Treatment with polymer after infection. HeLa cells grown in 96-well plates were infected with HRV2. 30 minutes after infection medium containing Iota-Carrageenan at the concentrations indicated at the x-axis was added. Plates were incubated at 37°C until cells in the control (no polymer added) showed >90% damage. Cell proliferation was determined with an XTT-assay. OD values (492 nm) obtained from mock infected cells (compare x-axis) were set to 100%, and the viability of cells infected in the absence of polymer was set to 0% (y-axis). Black triangles indicate an amount of input virus of 0,01 TCID₅₀/cell, black diamonds indicate 0,1 TCID₅₀/cell and black squares indicate 1 TCID₅₀/cell. A representative experiment is shown.