Figure 1

Compartmentalization of HCV proteins produced in HeLa cells infected with VT7-HCV _7.9 . Subconfluent HeLa cells uninfected or infected at 5 PFU/cell with the recombinant VT7-HCV7.9 in the presence (+) or absence (-) of the inducer IPTG, were fixed at 24 h p.i, labelled with the corresponding primary antibody followed by the appropriate fluorescent secondary antibody and visualized by confocal microscopy. The antibodies or reagents used were Hα HCV to detect HCV proteins; Topro-3 to detect DNA; Rα Giantin to detect the Golgi complex (A); Rα Calnexine to detect the endoplasmatic reticulum (B) and Mitotracker Deep Red 633 to detect mitochondria (C). The co-localization is shown with a higher resolution in the white square.