Figure 1From: Universal primers for HBV genome DNA amplification across subtypes: a case study for designing more effective viral primersOutput of BxB illiminated in ClustalX software. When the ratio of the most frequently presented nucleotide is larger than current cutoff value, the program outputs this nucleotide, otherwise outputs a '-'. The cutoff was set to (0.05, 1), and the step length is 0.05. The frequency is listed in the left box and the nucleotides are in the right box.Back to article page