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Table 4 Amplification of an under-represented LCV species in the presence of two over-represented, closely related LCV species

From: Discovery of herpesviruses in multi-infected primates using locked nucleic acids (LNA) and a bigenic PCR approach

  

Viral templates added to the PCR reaction

Copy number of added template

Pan-herpes PCR detection limit (template copy number)

Ratio between added template concentration and the detection limit

LNA added

Expected virus detection

Detected virus

A

1

EBV

10.000

10.000

1

-

  
 

2

GgorLCV1

100

10

10

GgorLCV1

EBV

EBV

 

3

CalHV3

10

1

10

CalHV3

  

B

1

EBV

100.000

10.000

10

EBV

  
 

2

GgorLCV1

10

10

1

-

GgorLCV1

GgorLCV1

 

3

CalHV3

10

1

10

CalHV3

  

C

1

EBV

100.000

10.000

10

EBV

  
 

2

GgorLCV1

100

10

10

GgorLCV1

CalHV3

CalHV3

 

3

CalHV3

1

1

1

-

  

D

1

EBV

10.000

10.000

1

-

  
 

2

GgorLCV1

1000

10

100

GgorLCV1

EBV

EBV

 

3

CalHV3

100

1

100

CalHV3

  

E

1

EBV

1000.000

10.000

100

EBV

  
 

2

GgorLCV1

10

10

1

-

GgorLCV1

GgorLCV1

 

3

CalHV3

100

1

100

CalHV3

  

F

1

EBV

1000.000

1.000

100

EBV

  
 

2

GgorLCV1

1000

10

100

GgorLCV1

CalHV3

GgorLCV1

 

3

CalHV3

1

1

1

-

  
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Virology Journal

ISSN: 1743-422X