Figure 2

Yeast two-hybrid analysis. (A) Representative plates showing homotypic interactions of the R5-Vpu protein. The first panel shows the template for the remaining panels that in turn show transformants streaked in each section of the indicated plates. Growth is seen as light streaks on a dark background, except in the β-galactosidase filter assay where signal is seen as dark streaks on a light background. (B) Complete results for the entire screen using NL-Vpu and R5-Vpu full-length, transmembrane domain and cytoplasmic domain fusions to the Gal4 protein DNA-binding domain (BD) or activation domain (AD). Growth (+) or no growth (-) of transformants on various media is shown. LTH-3AT represents growth on SDLeu^- Trp^-His^- plates containing 20 mM 3-amino-1,2,3-triazole. The β-galactosidase filter assay results are indicated as + or - and the liquid β-galactosidase assay values are shown in parentheses as an average of two independent measurements. Various negative and positive controls are also shown.