Figure 2From: Interaction between Bluetongue virus outer capsid protein VP2 and vimentin is necessary for virus egressDistribution of VP2 within infected and transfected Vero cells by fluorescence microscopy. A) Cells infected with BTV-10, B-E) transfected cells expressing B) VP2, C) VP2-GFP, D) GFP-VP2 and E) GFP only. VP2 in A and B were detected with anti VP2 monoclonal antibody (rabbit) and either FITC (A) or TRITC (B) conjugated secondary antibody. C-E were visualised based on GFP fluorescence. Expression of full-length, tagged VP2 variants was confirmed by western blot using an anti-GFP antibody (F).Back to article page