Figure 7From: Differential inhibition of human cytomegalovirus (HCMV) by toll-like receptor ligands mediated by interferon-beta in human foreskin fibroblasts and cervical tissueExpression of TLR by HFF and Ectocervical tissue. Expression of TLR2, TLR3, TLR4 and TLR9 in cells and tissue was assessed by reverse-transcription PCR. mRNA was isolated from THP-1 monocytic cells, HFF and tissue and reverse transcribed to create cDNA which was subjected to PCR using primers for each of the TLR as well as for GAPDH.Back to article page